Physiol. Genomics Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 20: 188-194, 2005. First published November 9, 2004; doi:10.1152/physiolgenomics.00157.2004
1094-8341/05 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Tables
Right arrow All Versions of this Article:
20/2/188    most recent
00157.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blomberg, L. A.
Right arrow Articles by Zuelke, K. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blomberg, L. A.
Right arrow Articles by Zuelke, K. A.
Received 22 July 2004; accepted in final form 5 November 2004.
Physiological Genomics 20:188-194 (2005)
1094-8341/05 $8.00 © 2005 American Physiological Society

Serial analysis of gene expression during elongation of the peri-implantation porcine trophectoderm (conceptus)

Le Ann Blomberg1, Ezhou L. Long1, Tad S. Sonstegard2, Curtis P. Van Tassell2, John R. Dobrinsky1 and Kurt A. Zuelke1

1 Biotechnology and Germplasm Laboratory, United States Department of Agriculture Agricultural Research Service, Beltsville, Maryland
2 Bovine Functional Genomics Laboratory, United States Department of Agriculture Agricultural Research Service, Beltsville, Maryland

Conceptus loss during the preimplantation and early postimplantation period hinders the efficiency of swine reproduction. Significant conceptus loss occurs during trophectoderm elongation between gestational day 11 (D11) and day 12 (D12). Elongation of the porcine conceptus is a key stage of development during which maternal recognition of pregnancy, initial placental development, and preparation for implantation occurs. The objective of this study was to establish comparative transcriptome profiles of D11 ovoid and D12 filamentous conceptuses and thereby identify temporally regulated genes essential for developmental progression during conceptus elongation. Serial analysis of gene expression (SAGE) libraries were constructed from in vivo derived ovoid and filamentous swine conceptuses to yield a total of 42,389 tags (ovoid) and 42,391 tags (filamentous) representing 14,464 and 13,098 putative unique transcripts, respectively. Statistical analysis of tag frequencies revealed the differential expression of 431 tags between libraries (P < 0.05). Nucleotide sequence alignment searches on public databases provided SAGE tag annotation and gene ontology assignments. Comparisons between the SAGE profiles of ovoid and filamentous conceptuses revealed increased expression of key genes in the steroidogenesis [cytochrome P-450scc (CYP11A1), aromatase (CYP19A), and steroidogenic acute regulatory protein (STAR)] and oxidative stress response pathways [microsomal glutathione S-transferase 1 (MGST1) and copper-zinc superoxide dismutase (SOD1)]. Differential expression of these genes in the steroidogenic and oxidative stress response pathways was confirmed by real-time PCR. These results validate the utility of SAGE in the pig and establish an initial model linking gene expression profiles at the pathway level with phenotypic progression from ovoid to filamentous stages of conceptus development.

preimplantation development; gene regulation; transcript profiles; serial analysis of gene expression




This article has been cited by other articles:


Home page
 J ANIM SCIHome page
J. R. Miles, B. A. Freking, L. A. Blomberg, J. L. Vallet, and K. A. Zuelke
Conceptus development during blastocyst elongation in lines of pigs selected for increased uterine capacity or ovulation rate
J Anim Sci, September 1, 2008; 86(9): 2126 - 2134.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
B. Xu, D. Geerts, K. Qian, H. Zhang, and G. Zhu
Myeloid ecotropic viral integration site 1 (MEIS) 1 involvement in embryonic implantation
Hum. Reprod., June 1, 2008; 23(6): 1394 - 1406.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
L. Blomberg, K. Hashizume, and C. Viebahn
Blastocyst elongation, trophoblastic differentiation, and embryonic pattern formation
Reproduction, February 1, 2008; 135(2): 181 - 195.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
A. Waclawik and A. J Ziecik
Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pig
J. Endocrinol., September 1, 2007; 194(3): 499 - 510.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
B. A. Freking, K. A. Leymaster, J. L. Vallet, and R. K. Christenson
Number of fetuses and conceptus growth throughout gestation in lines of pigs selected for ovulation rate or uterine capacity
J Anim Sci, September 1, 2007; 85(9): 2093 - 2103.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
K. F. Rodriguez, L. A. Blomberg, K. A. Zuelke, J. R. Miles, J. E. Alexander, and C. E. Farin
Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression
Physiol Genomics, November 21, 2006; 27(3): 318 - 327.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the American Physiological Society.