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This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 34/1/112    most recent 00239.2007v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Han, E.-S. Articles by Richardson, A. PubMed PubMed Citation Articles by Han, E.-S. Articles by Richardson, A.

The in vivo gene expression signature of oxidative stress

¹ Department of Biological Science, University of Tulsa, Tulsa, Oklahoma
² Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio
³ Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio
⁴ Center for Epidemiology and Biostatistics, University of Texas Health Science Center at San Antonio
⁵ Geriatric Research Education and Clinical Center, South Texas Veterans Health Care System, San Antonio, Texas
⁶ Department of Pediatrics, University of California, San Francisco, California
⁷ Departments of Pharmacology and Medicine, Vanderbilt University, Nashville, Tennessee

How higher organisms respond to elevated oxidative stress in vivo is poorly understood. Therefore, we measured oxidative stress parameters and gene expression alterations (Affymetrix arrays) in the liver caused by elevated reactive oxygen species induced in vivo by diquat or by genetic ablation of the major antioxidant enzymes CuZn-superoxide dismutase (Sod1) and glutathione peroxidase-1 (Gpx1). Diquat (50 mg/kg) treatment resulted in a significant increase in oxidative damage within 3–6 h in wild-type mice without any lethality. In contrast, treatment of Sod1^–/– or Gpx1^–/– mice with a similar concentration of diquat resulted in a significant increase in oxidative damage within an hour of treatment and was lethal, i.e., these mice are extremely sensitive to the oxidative stress generated by diquat. The expression response to elevated oxidative stress in vivo does not involve an upregulation of classic antioxidant genes, although long-term oxidative stress in Sod1^–/– mice leads to a significant upregulation of thiol antioxidants (e.g., Mt1, Srxn1, Gclc, Txnrd1), which appears to be mediated by the redox-sensitive transcription factor Nrf2. The main finding of our study is that the common response to elevated oxidative stress with diquat treatment in wild-type, Gpx1^–/–, and Sod1^–/– mice and in untreated Sod1^–/– mice is an upregulation of p53 target genes (p21, Gdf15, Plk3, Atf3, Trp53inp1, Ddit4, Gadd45a, Btg2, Ndrg1). A retrospective comparison with previous studies shows that induction of these p53 target genes is a conserved expression response to oxidative stress, in vivo and in vitro, in different species and different cells/organs.

oxidative stress; gene expression; p53 target genes; Sod1; Gpx1