Physiol. Genomics Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Genomics 7: 27-34, 2001;
1094-8341/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (34)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by CAGNEY, G.
Right arrow Articles by FIELDS, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by CAGNEY, G.
Right arrow Articles by FIELDS, S.
Received 14 March 2001; accepted in final form 1 August 2001.
Physiological Genomics 7:27-34 (2001)
1094-8341/01 $5.00 © 2001 American Physiological Society

Two-hybrid analysis of the Saccharomyces cerevisiae 26S proteasome

GERARD CAGNEY1, PETER UETZ1 and STANLEY FIELDS1,2

1 Departments of Genetics and Medicine
2 Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195-7360

A two-hybrid screen against an activation domain array of Saccharomyces cerevisiae proteins was carried out for 31 yeast proteasome proteins. Fifty-five putative interactions were identified: 21 between components of the proteasome complex and 34 between proteasome proteins and other proteins. Many of these latter interactions involved either proteins of the ubiquitin pathway, cell cycle proteins, protein kinases or a translation initiation factor subunit. The role of eleven proteins associated with proteasome function by these screens was analyzed by examining the corresponding deletion strains for temperature sensitivity and canavanine sensitivity and for the stability of a ubiquitin-ß-galactosidase fusion protein. These assays additionally implicated three proteins, Bim1, Ump1, and YKL171W, in proteasome function. This study demonstrates the utility of genome-wide two-hybrid assays as an entry point for the further analysis of a large protein complex.

protein degradation; protein interactions; ubiquitin




This article has been cited by other articles:


Home page
 JCBHome page
B. Medicherla and A. L. Goldberg
Heat shock and oxygen radicals stimulate ubiquitin-dependent degradation mainly of newly synthesized proteins
J. Cell Biol., August 25, 2008; 182(4): 663 - 673.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
T. Chiang, N. Li, S. Orchard, S. Kerrien, H. Hermjakob, R. Gentleman, and W. Huber
Rintact: enabling computational analysis of molecular interaction data from the IntAct repository
Bioinformatics, April 15, 2008; 24(8): 1100 - 1101.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Ivantsiv, L. Kaplun, R. Tzirkin-Goldin, N. Shabek, and D. Raveh
Unique Role for the UbL-UbA Protein Ddi1 in Turnover of SCFUfo1 Complexes.
Mol. Cell. Biol., March 1, 2006; 26(5): 1579 - 1588.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. D. Miles, J. Jakovljevic, E. W. Horsey, P. Harnpicharnchai, L. Tang, and J. L. Woolford Jr
Ytm1, Nop7, and Erb1 Form a Complex Necessary for Maturation of Yeast 66S Preribosomes
Mol. Cell. Biol., December 1, 2005; 25(23): 10419 - 10432.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Heink, D. Ludwig, P.-M. Kloetzel, and E. Kruger
From The Cover: IFN-{gamma}-induced immune adaptation of the proteasome system is an accelerated and transient response
PNAS, June 28, 2005; 102(26): 9241 - 9246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Strange
The end of "naive reductionism": rise of systems biology or renaissance of physiology?
Am J Physiol Cell Physiol, May 1, 2005; 288(5): C968 - C974.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Isono, N. Saito, N. Kamata, Y. Saeki, and A. Toh-e
Functional Analysis of Rpn6p, a Lid Component of the 26 S Proteasome, Using Temperature-sensitive rpn6 Mutants of the Yeast Saccharomyces cerevisiae
J. Biol. Chem., February 25, 2005; 280(8): 6537 - 6547.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Dong, W. Chen, A. Welford, and A. Wandinger-Ness
The Proteasome {alpha}-Subunit XAPC7 Interacts Specifically with Rab7 and Late Endosomes
J. Biol. Chem., May 14, 2004; 279(20): 21334 - 21342.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Kaplun, Y. Ivantsiv, A. Bakhrat, and D. Raveh
DNA Damage Response-mediated Degradation of Ho Endonuclease via the Ubiquitin System Involves Its Nuclear Export
J. Biol. Chem., December 5, 2003; 278(49): 48727 - 48734.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. H. Glickman and A. Ciechanover
The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction
Physiol Rev, April 1, 2002; 82(2): 373 - 428.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online