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Physiol. Genomics 2: 143-147, 2000;
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Received 21 December 1999; accepted in final form 2 March 2000.
Physiological Genomics 2:143-147 (2000)
1094-8341/00 $5.00 © 2000 American Physiological Society

Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes

JANET A. WARRINGTON, ARCHANA NAIR, MAMATHA MAHADEVAPPA and MAYA TSYGANSKAYA

Affymetrix, Inc., Santa Clara, California 95051

Warrington, Janet A., Archana Nair, Mamatha Mahadevappa, and Maya Tsyganskaya. Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes. Physiol Genomics 2: 143–147, 2000.—Gene expression levels of about 7,000 genes were measured in 11 different human adult and fetal tissues using high-density oligonucleotide arrays to identify genes involved in cellular maintenance. The tissues share a set of 535 transcripts that are turned on early in fetal development and stay on throughout adulthood. Because our goal was to identify genes that are involved in maintaining cellular function in normal individuals, we minimized the effect of individual variation by screening mRNA pooled from many individuals. This information is useful for establishing average expression levels in normal individuals. Additionally, we identified transcripts uniquely expressed in each of the 11 tissues.

housekeeping genes; DNA chips; human gene expression; cellular maintenance genes




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