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Cover Caption:
Cover: X-Gal staining of brains derived from transgenic mice harboring
a Tie2 promoter-tetracycline-on cassette, and a nuclear localized form
of 
-galactosidase under control of a tetracycline-responsive
promoter. This system allows for regulated, endothelial-selective
expression of the nl-Gal transgene in response to exogenous
doxycycline. The brain on the right was derived from an animal
treated with doxycycline, and the brain on the left was from an
untreated animal. The X-Gal staining is seen in the nuclei of the
endothelial cells lining the cerebral vessels on the ventral surface of
the brain, in the doxycycline-treated animal. This system can be readily
adapted to selectively express any transgene of interest in the vascular
endothelium. For further discussion, see the article by
Teng PI, DiChiara MR, Kömüves LG, Abe K,
Quertermous T, and Topper JN. Inducible and selective transgene
expression in murine vascular endothelium. Physiol Genomics 11: 99-107,
2002. First published September 11, 2002;
10.1152/physiolgenomics.00059.2002.
Copyright © 2003 by the American Physiological Society.
